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KMID : 0380219940270010064
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Journal of Biochemistry and Molecular Biology
1994 Volume.27 No. 1 p.64 ~ p.68
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Resistance Mechanism of Clinically Isolated Ofloxacin Resistant Pseudomonas aeruginosa to HK3140-A New Fluoroquinolone
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Abstract
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Abstract:
@EN In this study, the resistance mechanism of Pseudomonas aeruginosa to HK3140(Fig. 1)-a new fluoroquinole-was characterized using a HK140 susceptible strain and a HK3140 strain which was selected among clinically isolated ofloxacin resistant P.
aeruginosa strains during ofloxacin therapy. The susceptible and the resistant strains showed a remarkable difference in their outer membrane protein profiles. HK3140 concentrations inside the susceptible and the resistant strains were increased
in the
presence of carbonylcyanide m-chlorophenyl hydrazone (CCCP). HK3140 concentrations inside the resistant strain was increased in the presence of KNO3, too. These results were confirmed by measuring the in vivo DNA synthesis in the presence of CCCP
and
KNO3, CCCP decreased the in vivo DNA synthesis in both stains while KNO3 decreased DNA synthesis only in the resistant strain. When DNA synthesis in the permeabilized cells were measured in the presence of HK3140, the amount of Dna synthesized in
the
permeabilized resistant strain was not greater a than that in the susceptible strain. Results suggested that the resistance of P. aeruginosa to HK3140 is a result from a change in permeability from changes in outer membrane proteins and an
occurrence of
a new ATPase dependent efflux system in addition to an intrinsic proton gradient dependent efflux system, but jfrom a change in the DNA gyrase.
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KEYWORD
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